The RTmfm expert group on “Transparization of thick samples” has developed this guide to share and inform the scientific community about its expertise in the transparization of thick samples. You will find tools that will enable you to conduct your own experiments in sample transparization and fluorescence microscopy.
In 2022, actions will focus on two main areas:
Communication, technology monitoring, and diversification of dissemination, training, and sharing tools.
Communication will be handled through the new website recently set up by RTmFm. The Beginner's Kit will be updated in terms of methodology, particularly with regard to the possibility of combining transparency methods using organic solvents and aqueous media. If possible, links to practical video workshops will be added as soon as the videos are filmed. Interactive mapping of experts: the transfer to the new site will require an update of the entire IT architecture. Training: This will take the form of webinars and educational videos/technology workshops. The equipment provided by MITI for video recording will be tested. A script and initial recording schedule will be drawn up and distributed to members over the coming weeks so that they can be incorporated into the beginner's kit and/or the website as and when they become available. A themed day for mapping experts will be organized as a webinar with the aim of promoting interaction between experts and improving technology monitoring.
Promoting know-how and expertise: Standardization and Reference Frameworks
We will continue to experiment with standardizing procedures in line with transparency approaches. Comparative experiments on different types of microscopes carried out in 2021 will be continued to assess differences in relation to immersion in water. This project will interact with the RTmFm's 3M Working Group to validate the relevance of the choice of standardization strategies for “reference” measurements. The preparation of two biological models (zebrafish and mouse central nervous system) to apply the same parameters given by the metrological tool is planned. The objective is to be able to publish a reference guide for service platforms in the medium term, enabling the methodological and technological transfer of transparent biological sample acquisition on different microscopy systems.
To learn more about the actions of the Transparency WG, you can download the “WG Flyer” or download the presentation from the 2021 Conference.
Romina D’Angelo | Coordinator 2018-2024 | SigDYN-CRCT, Toulouse
Pierre Affaticati | TEFOR, Saclay
Chloé Chaumeton | Current co-coordinator | IBPS, Paris
Katia Belcram | IJPB OV-PCIV, Versailles
Laurence Dubreil | APEX_PAnTher UMR703 INRAE/Oniris, Nantes
David Godefroy | Co-coordinator 2018-20 | NorDic U1239, Rouen
François Michel | INMED UMR 1249 INSERM, Université Aix-Marseille
Matthieu Simion | MIMA2, Jouy-en-Josas
Jérémie Teillon | Current co-coordinator | BIC, Bordeaux
Tudor MANOLIU | PFIC, Gustave Roussy, Villejuif